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KMID : 0381919990290040451
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Korean Journal of Microscopy
1999 Volume.29 No. 4 p.451 ~ p.457
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Ultrastructural Study of Drosophila Ocellar Visual System by Osmium Impregnation
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Yoon Chun-Sik
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Abstract
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Ultrastructure of adult Drosophila ocellus was compared with conventional electron microscopic method and osmium impregnation. When osmium impregnation was applied, some organelles of cells were strongly stained. Especially, subrhabdomeric cisternae (SRC) were strongly stained and showed network-iike structure as in compound eye. Other organelles including SSC, ER, nuclear envelope, pigment granules and mitochondria were also strongly stained. These organelles are known as a general calcium ion reservoir. In conclusion, the strong effect of light and shade by osmium impregnation was regarded as a result of strong binding between calcium ion and osmium tetroxide. Thus, we agree to the opinion that osmium impregnation is very useful methods to the comparative morphology of cell organelles.
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KEYWORD
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Drosophila, Ocellar visual system, Osmium impregnation
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